簡要描述：BioGems CD28單克隆抗體，SAFIRE 純化級:
BioGems的SAFIRE （Sodium Azide Free Immensely Reduced Endotoxins） 類產(chǎn)品是BioGems品牌下的功能性抗體產(chǎn)品，經(jīng)特殊工藝生產(chǎn)、純化，可用于免疫細(xì)胞功能的激活、阻斷、中和研究。
SAFIRE純化CD28單抗 （clone: CD28.2）是 T細(xì)胞激活、增殖的輔助刺激分子。

Anti-Human CD28 SAFIRE Purified   CD28單抗

產(chǎn)品英文名稱：Anti-Human CD28 SAFIRE Purified

產(chǎn)品中文名稱：CD28單克隆抗體/抗人CD28抗體

BioGems CD28單抗特點(diǎn)

極低的內(nèi)毒素含量，低于0.01EU/μg蛋白 

不含疊氮鈉，適用于體內(nèi)或體外實(shí)驗(yàn)

高蛋白純度

應(yīng)用 BioGems CD28單抗適用細(xì)胞：T細(xì)胞

作用機(jī)理 CD28單克隆抗體特異性地與人類44 kDa同源二聚體跨膜糖蛋白CD28結(jié)合，CD28在大多數(shù)成熟的T淋巴細(xì)胞、漿細(xì)胞和胸腺細(xì)胞的表面表達(dá)。CD28是B7-1(CD80)和B7-2(CD86)的配體，T淋巴細(xì)胞的共刺激分子，并能增強(qiáng)T和B淋巴細(xì)胞間的相互作用。據(jù)報道，T淋巴細(xì)胞刺激產(chǎn)生IL-2依賴于單克隆抗體,這表明CD28分子呈現(xiàn)出具有不同功能的亞區(qū)域。CD28單克隆抗體誘導(dǎo)Ca2+流入Jurkat T淋巴細(xì)胞。其他研究表明CD28與信號轉(zhuǎn)導(dǎo)有關(guān)。

Description

The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.

質(zhì)量控制

BioGems CD28單克隆抗體經(jīng)過親和層析純化。

內(nèi)毒素水平：蛋白質(zhì)小于0.01 EU/µg，鱟試劑法檢測。

訂購信息        

紅榮微再代理的BioGems CD3/CD28單抗正進(jìn)行年末*活動：買二送一（四折之后），并贈送抗霾禮盒

Biogems是Peprotech的抗體品牌。CD3/CD28單抗BioGems*現(xiàn)正進(jìn)行4折*。

CD3  500ug 現(xiàn)價4130元 *(4折) 1652元

CD28  500ug 現(xiàn)價3640元 *(4折) 1456元

BioGems的SAFIRE (Sodium Azide Free Immensely Reduced Endotoxins) 類產(chǎn)品是BioGems品牌下的功能性抗體產(chǎn)品，經(jīng)特殊工藝生產(chǎn)、純化，具有諸多特點(diǎn)，可用于免疫細(xì)胞功能的激活、阻斷、中和研究，是腫瘤免疫研究的重要工具。

SAFIRE純化CD3單抗 (clone: OKT3)可以與TCR復(fù)合物結(jié)合，激活T細(xì)胞；SAFIRE純化CD28單抗 (clone: CD28.2)是 T細(xì)胞激活、增殖的輔助刺激分子。

紅榮微再*

CD3 CD28單抗買二送一！

在4折基礎(chǔ)上再加優(yōu)惠。

買二：CD3、CD28任選兩支。

送一：CD3、CD28任選一支。

贈送抗霾禮盒

抗霾禮盒

易可濾掛機(jī)空調(diào)伴侶防PM2.5空調(diào)過濾

易可濾防霧霾過濾PM2.5可更換式口罩

價值600多元

活動時間：2017年12月15日 - 2018年3月31日

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：1500 1904 520。紅榮微再-客服:  經(jīng)銷商專員

CD3/CD28功能性抗體應(yīng)用：CAR-T及免疫細(xì)胞研究

CAR-T介紹

       通過增強(qiáng)免疫系統(tǒng)對腫瘤的反應(yīng)來治療癌癥的腫瘤免疫療法被認(rèn)為具有巨大的臨床應(yīng)用前景。其中的過繼性免疫療法—CAR-T療效 法，正受到科研與臨床的極大關(guān)注，已有多家研究機(jī)構(gòu) (如Baylor, Fred Hutchinson, NCI)展開臨床研究，而今年FDA更是已經(jīng)批準(zhǔn)了諾華和Kite兩款CAR-T治療藥物。

Anti-Human CD28 SAFIRE Purified 說明書

Catalogue Number : 10311-25

RUO: For Research Use Only. Not for use in diagnostic procedures.

Product Information

Clone: CD28.2

Format/Conjugate: SAFIRE Purified

Concentration: 2 mg/mL

Reactivity: Human

Laser: Not Applicable

Peak Emission: Not Applicable

Peak Excitation: Not Applicable

Filter: Not Applicable

Brightness (1=dim,5=brightest): Not Applicable

Isotype: Mouse IgG1, kappa

Formulation: Phosphate-buffered aqueous solution, ph7.2.

Storage:Product should be kept at 2-8°C and protected from prolonged exposure to light.

Applications: FC, FA, IHC, IP

Description

The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28,expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80)and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.

Preparation & Storage

The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity

chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Application Notes

The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. It is recommended that the reagent be titrated for optimal performance for each application.

References

1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

2.Nunès, J., Klasen, S., Ragueneau, M., Pavon, C., Couez, D., Mawas, C., ... ; Olive, D. (1993). CD28 mAbs with distinct binding properties differ in

their ability to induce T cell activation: analysis of early and late activation events.International immunology,;5(3), 311-315.

3.Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., ; Vaslin, B. (2007). FoxP3+ CD25+ CD8+ T-cell induction during primary

simian immunodeficiency virus infection in cynomolgus macaques correlates with low CD4+ T-cell activation and high viral load.;Journal of virology,;81(24),

13444-13455.

歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司  ：1500 1904 520。紅榮微再-客服:  經(jīng)銷商專員