簡要描述：iSTEM小鼠胚胎干細胞培養(yǎng)基是一款的小鼠胚胎干細胞維持培養(yǎng)基，還可以從難獲得ES細胞的小鼠品系中獲取小鼠ES細胞系。培養(yǎng)基添加3種小分子抑制劑阻斷分化，擴增性能相比添加了LIF和BMP的培養(yǎng)基更*。

 華雅旗下紅榮微再授權(quán)代理（上海）TAKARA CellArtis，Rubicon產(chǎn)品 

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英文名稱：Cellartis iSTEM Embryonic Stem Cell Culture medium

中文名稱：Cellartis iSTEM小鼠胚胎干細胞培養(yǎng)基

用途：小鼠胚胎干細胞維持培養(yǎng)

特別應(yīng)用：從難獲得ES細胞的小鼠品系中獲取小鼠ES細胞系

保存：-20ºC

iSTEM是一款的小鼠胚胎干細胞維持培養(yǎng)基。該款產(chǎn)品包含三種小分子抑制物，從而阻斷干細胞分化的信號通路，同時維持細胞的多能干性（pluripotency）。 iSTEM可以被應(yīng)用于難以獲得ES細胞的小鼠品系，獲取到ES細胞系(1)。

iSTEM培養(yǎng)基中的小鼠ES細胞與在添加了Leukemia Inhibitory Factor (LIF) 和 serum/Bone Morphogenetic Protein (BMP)的培養(yǎng)基的細胞相比，增殖速度加倍(1)。另外，在iSTEM中培養(yǎng)的小鼠ES細胞產(chǎn)生細胞聚落（clone）的能力顯著提高（enhanced clonogenicity）,從而可以在單細胞鋪板后（single cell deposition），產(chǎn)生更多的干細胞克隆。

iSTEM is a compley defined cell culture medium for deriving, maintaining, and propagating mouse embryonic stem (ES) cells. This media formulation captures mouse ES cells in their basal state by blocking the inductive pathways of differentiation with selective small molecule inhibitors. The medium contains three small molecule inhibitors that block differentiation-inducing signals and promote cell survival, enabling maintenance of pluripotency without the need for additional stimulatory cytokines. iSTEM medium can also be used to derive mouse ES lines from strains that have proven refractory to ES cell derivation under standard conditions (1).

Mouse ES cells cultured in iSTEM medium continually expand at a doubling rate that is comparable to that of cells cultured in the presence of Leukemia Inhibitory Factor (LIF) and serum/Bone Morphogenetic Protein (BMP) (1). In addition, culture in iSTEM medium enhances clonogenicity; a higher frequency of pluripotent colonies are established after single cell deposition.

產(chǎn)品特點

*確定的配方：無血清，無飼養(yǎng)層和無細胞因子

使用選擇性小分子抑制劑來阻斷分化信號并將ES細胞維持在多能狀態(tài)

使用iSTEM培養(yǎng)基，小鼠ES細胞可以有效地維持并以≥99％的純度擴增

Compley defined formulation: serum-free, feeder-free, and cytokine-free

Uses selective small-molecule inhibitors to block differentiation signals and maintain ES cells in a pluripotent state

Using iSTEM medium, mouse ES cells can be efficiently maintained and propagated at ≥99% purity

產(chǎn)品應(yīng)用

保持小鼠ES細胞的多能狀態(tài)

從難獲得ES細胞的(non-permissive)小鼠品系中獲取小鼠ES細胞系

Maintaining mouse ES cells in a pluripotent state

Deriving mouse ES cell lines from non-permissive mouse strains

組份：培養(yǎng)基+添加劑

100 ml medium

100 µl supplement

自備試劑：

For mouse ES cells

Use culture vessels precoated with 0.1% gelatin type A in PBS (at room temperature for 15 minutes or more), or precoated sequentially with 0.01%poly-L-ornithine hydrobromide (at 37℃ for 30 minutes or more), washing twice with PBS, followed by 10 μg/mL laminin in PBS (at 37℃ for 3 hours or more).

對于小鼠ES細胞

使用在PBS中預(yù)涂有0.1％明膠A型的培養(yǎng)容器（在室溫下15分鐘或更長時間），或者依次用0.01％聚-L-鳥氨酸氫溴酸鹽（在37℃下30分鐘或更多）預(yù)涂覆，用PBS洗滌兩次 ，然后用PBS中的10μg/ mL層粘連蛋白（37℃下3小時以上）。

保存：Upon receipt, store at -20℃ until ready to use. When stored under these conditions,the product is stable for 12 months from the date of manufacture (see label).Once thawed, store at 4℃ and use within 2 weeks.This product is light sensitive, and should be protected from light.

參考文獻：

Ying, Q. L. et al. (2008) The ground state of embryonic stem cell self-renewal. Nature 453:519–523.

Cellartis 小鼠/大鼠胚胎干細胞培養(yǎng)基系列：成分確定、無血清培養(yǎng)基

GS2-M® 小鼠胚胎干細胞和多能誘導(dǎo)干細胞培養(yǎng)基，無飼養(yǎng)層，不依賴LIF

iSTEM™ 小鼠胚胎干細胞培養(yǎng)基，難獲得ES細胞小鼠

GS1-R® 大鼠多能干細胞培養(yǎng)基，需要飼養(yǎng)層，胚胎干細胞和胚胎生殖細胞（EG）

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導(dǎo)技術(shù)和ES細胞，ips細胞，分化細胞等干細胞相關(guān)產(chǎn)品。

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