簡要描述：Definitive Endoderm Cells內(nèi)胚層干細胞是從Cellartis 人iPS細胞株ChiPSC18經(jīng)體外誘導(dǎo)分化得來。該產(chǎn)品中，大于90%的細胞顯示表達SOX17蛋白?？捎糜谶M一步分化成為肝細胞或胰臟細胞。

 華雅旗下紅榮微再授權(quán)代理（上海）TAKARA CellArtis，Rubicon產(chǎn)品  現(xiàn)貨數(shù)量有限 詳詢 手機：1500 1904 520 紅榮微再-客服: 經(jīng)銷商專員：

Cellartis定型內(nèi)胚層干細胞

產(chǎn)品英文名稱：Cellartis Definitive Endoderm Cells (from ChiPSC18)

產(chǎn)品中文名稱：Cellartis定型內(nèi)胚層干細胞

適合用于進一步誘導(dǎo)分化成為肝臟細胞類型或是胰臟細胞類型。

Cellartis定型內(nèi)胚層干細胞（DE cell）是從hiPS細胞株ChiPSC18經(jīng)體外誘導(dǎo)分化得來。該產(chǎn)品中，大于90%的細胞顯示表達SOX17蛋白。該產(chǎn)品為低溫冷凍保存的細胞懸浮形式。本產(chǎn)品適合用于進一步誘導(dǎo)分化成為肝臟細胞類型或是胰臟細胞類型。特別地，Cellartis的肝臟細胞誘導(dǎo)分化試劑盒（Cellartis Hepatocyte Differentiation Kit，Cat. No. Y30050）適合用于DE向肝臟細胞類型的誘導(dǎo)分化工作。

在產(chǎn)生Cellartis定型內(nèi)胚層細胞（來自ChiPSC18）的分化過程中，通過RT-qPCR測量的mRNA表達譜顯示內(nèi)胚層標志物SOX17，F(xiàn)OXA2，F(xiàn)ZD5，CXCR4，HHEX和SOX7的預(yù)期時間表達; 中胚層標記Brachyury; 和多能標記OCT4。

Cellartis Definitive Endoderm Cells (from ChiPSC18) are human definitive endoderm (DE) cells derived from the human induced pluripotent stem (hiPS) cell line ChiPSC18. The hiPS cells have been differentiated into definitive endoderm cells in vitro to generate a population of >90% SOX17-protein-expressing cells; they are then dissociated into a cell suspension and cryopreserved. These DE cells are suitable for further differentiation into hepatocytes or pancreatic endoderm cells. To differentiate the DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050).

During the differentiation process that creates Cellartis Definitive Endoderm Cells (from ChiPSC18), the mRNA expression profile—as measured by RT-qPCR—shows the expected temporal expression of endodermal markers SOX17, FOXA2, FZD5, CXCR4, HHEX, and SOX7; mesoendodermal marker Brachyury; and pluripotency marker OCT4. See the Images & Data tab, below.

產(chǎn)品特點:

具有> 90％SOX17陽性細胞的定形內(nèi)胚層細胞

可用于下一步分化內(nèi)胚層起源的下級分化細胞，如肝細胞或胰腺內(nèi)胚層

Definitive endoderm cells with >90% SOX17-positive cells

Can be used for generating further-specified cells of endodermal origin, such as hepatocytes or pancreatic endoderm

組份：

Cellartis Definitive Endoderm Cells (from ChiPSC18) (Cat. No. Y10040)

保存：Cellartis Definitive Endoderm Cells (from ChiPSC18) should be stored at ≤ –150°C. Under

recommended storage conditions, the cells can be stored for up to one year from the date of receipt.

Culture thawed Cellartis Definitive Endoderm Cells (from ChiPSC18) at 37°C ± 1°C, 5% CO2 and>90% humidity.

產(chǎn)品性能：

Immunocytochemistry of Cellartis Definitive Endoderm Cells (from ChiPSC18). Cells were thawed, cultured for two days, fixed, and stained for the pluripotency marker OCT4, the definitive endoderm marker SOX17, and the nuclear marker DAPI. Results show that more than 90% of the cell population are definitive endoderm cells and have lost pluripotency.

相關(guān)產(chǎn)品：

Cellartis DEF-CS™ 500 COAT-1 (Takara Bio, Cat. No. Y30012)

iMatrix-511 (Takara Bio, Cat. No. T303)

iMatrix-511 (Takara Bio, Cat. No. T304)

Cellartis Hepatocyte Differentiation Kit (Takara Bio, Cat. No. Y30050)

肝細胞培養(yǎng)完整解決方案

人iPS誘導(dǎo)分化成肝細胞全過程的完整試劑盒：Y30055（新產(chǎn)品）

人iPS來源肝細胞株及*培養(yǎng)基Y10133/Y10134/Y10135（新產(chǎn)品）

人原代肝臟細胞培養(yǎng)基 Y20020（新產(chǎn)品）

http://www.chem17.com/st321032/article_1496885.html

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營細胞治療和精準醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導(dǎo)技術(shù)和ES細胞，ips細胞，分化細胞等干細胞相關(guān)產(chǎn)品。

Cellartis-Y10040 Human Stem Cell Derived Definitive Endoderm Cells 及更多細胞培養(yǎng)基產(chǎn)品咨詢電話 手機1500 1904 520 紅榮微再-客服：  經(jīng)銷商專員：